Alpha-1 AG
Alpha-1 Acid Glycoprotein
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Cat. No.:
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KAI-021
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Type:
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Reagent
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Method:
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Immunoturbidimetric
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Format:
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Liquid
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Linearity:
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10 - 200 mg/dL
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Sample:
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Serum
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KIT COMPOSITION
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INTENDED USE
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For the quantitative determination of human alpha-1 acid glycoprotein (alpha-1 AG) in human serum by
immunoturbidimetric assay. Measurement of alpha-1 acid glycoprotein may aid in the diagnosis of
collagen (connective tissue) disorders, tuberculosis, infections, extensive malignancy, and diabetes.
FOR IN VITRO DIAGNOSTIC USE.
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REAGENT PREPARATION
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The reagents are ready to use. They do not need to be reconstituted.
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STORAGE AND HANDLING
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All reagents should be stored refrigerated (2-8°C). Return all reagents to
2-8°C promptly after use. Unopened reagents can be used for one year from the
date of manufacture, as indicated by the expiration date on the package and bottle
labels. Opened reagents can be used for one month if stored at 2-8°C.
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PERFORMANCE
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Precision
The precision for the
K-ASSAY ®
Alpha-1 AG assay was determined using packaged reagents, pooled human serum, and a Hitachi 717 analyzer.
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Precision - Within Run
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Sample I
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Sample II
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Sample III
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N = 20
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N = 20
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N = 20
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Mean = 32.5
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Mean = 92.3
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Mean = 233.7
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S.D. = 0.53
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S.D. = 0.6
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S.D. = 2.17
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C.V. = 1.6%
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C.V. = 0.7%
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C.V. = 0.9%
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Precision - Between Runs
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Sample I
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Sample II
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Sample III
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N = 10
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N = 10
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N = 10
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Mean = 54.6
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Mean = 104.7
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Mean = 198.6
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S.D. = 0.69
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S.D. = 0.93
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S.D. = 1.37
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C.V. = 1.27%
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C.V. = 0.89%
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C.V. = 0.69%
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Accuracy / Correlation
A comparison of the
K-ASSAY ®
Alpha-1 AG assay and a Binding Site Alpha-1 AG RID Test Kit was performed using a Hitachi 717. The test results provided the following data:
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y = 1.016x + 3.547
r = 0.995
n = 37
x = Binding Site Alpha-1 AG RID
y = K-ASSAY ® Alpha-1 AG assay
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x
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min
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=
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35
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y
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min
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=
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37
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max
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=
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195
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max
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=
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201
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mean
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=
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74
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mean
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=
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79
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Assay Range
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INTERFERENCE
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Ascorbic Acid :
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No interference up to 200 mg/dL
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Bilirubin C :
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No interference up to 20 mg/dL
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Bilirubin F :
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No interference up to 20 mg/dL
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Hemoglobin :
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No interference up to 500 mg/dL
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Intralipid :
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No interference up to 400 mg/dL
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