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Haptoglobin
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Cat. No.:
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KAI-022
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Type:
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Reagent
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Method:
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Immunoturbidimetric
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Format:
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Liquid
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Linearity:
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20 - 220 mg/dL
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Sample:
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Serum
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KIT COMPOSITION
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INTENDED USE
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For the quantitative determination of human haptoglobin in human serum by immunoturbidimetric assay.
Haptoglobin is a protein that binds hemoglobin, the oxygen-carrying pigment in red blood cells.
Measurement of haptoglobin may aid in the diagnosis of hemolytic diseases (diseases in which the
red blood cells rupture and release hemoglobin) relating to the formation of hemoglobin-haptoglobin
complexes and certain kidney diseases.
FOR IN VITRO DIAGNOSTIC USE.
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REAGENT PREPARATION
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The reagents are ready to use. They do not need to be reconstituted.
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STORAGE AND HANDLING
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All reagents should be stored refrigerated (2-8°C). Return all reagents to
2-8°C promptly after use. Unopened reagents can be used for one year from the
date of manufacture, as indicated by the expiration date on the package and bottle
labels. Opened reagents can be used for one month if stored at 2-8°C.
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PERFORMANCE
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Precision
The precision for the
K-ASSAY ®
Haptoglobin assay was determined using packaged reagents, pooled human serum, and a Hitachi 717 analyzer.
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Precision - Within Run
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Sample I
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Sample II
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N = 20
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N = 20
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Mean = 68.15
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Mean = 366
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S.D. = 0.88
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S.D. = 5.5
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C.V. = 1.3%
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C.V. = 1.5%
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Precision - Between Runs
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Sample I
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Sample II
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N = 8
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N = 8
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Mean = 85.9
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Mean = 225.6
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S.D. = 0.8
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S.D. = 1.4
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C.V. = 0.972%
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C.V. = 0.624%
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Accuracy / Correlation
A comparison of the
K-ASSAY ®
Haptoglobin assay and an Incstar Haptoglobin Test Kit was performed using a Hitachi 717. The test results provided the following data:
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y = 0.937x - 9.25
r = 0.993
n = 60
x = Incstar Haptoglobin Test Kit
y = K-ASSAY ® Haptoglobin assay
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x
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min
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=
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34
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y
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min
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=
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4
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max
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=
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266
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max
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=
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267
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mean
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=
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109
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mean
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=
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93
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Assay Range
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INTERFERENCE
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Bilirubin C :
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No interference up to 20 mg/dL
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Bilirubin F :
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No interference up to 20 mg/dL
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Intralipid :
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No interference up to 500 mg/dL
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Haptoglobin binds tightly to free hemoglobin. Use of hemolyzed samples is not
recommended as will have a negative effect on this assay's results.
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